Ecosyste.ms: Repos
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GitHub topics: transcriptomics-assembly
ReemaSingh/Transcriptomics-Assembly
De-novo Transcriptomics Assembly workflow for four Dictyostelium species (e.g.- Dictyostelium discoideum, Polysphondylium pallidum, Dictyostelium Lacteum and Dictyostelium Fasciculatum). This is the standard assembly workflow that should ideally work on any organism.Before normalizing first concatenate all RNAseq data across all samples into a single set of inputs to generate a single reference transcriptomics assembly. Combine all left reads in one file and all right reads in another file. In order to reduce the number, raw reads were normalized using in silico digital normalization implemented in trinity at 50X coverage. The reads were assembled with Trinity using kmer parameter of 25.
Language: Perl - Size: 26.4 KB - Last synced: 8 months ago - Pushed: over 3 years ago - Stars: 1 - Forks: 0
pengkenlim/LSTrAP-denovo
LSTrAP-denovo is an easy-to-use pipeline to assemble and annotate transcripts for species without genome / genomic annotations using publicly available RNA-seq data.
Language: Python - Size: 1.64 MB - Last synced: over 1 year ago - Pushed: over 1 year ago - Stars: 0 - Forks: 0
